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Comparative cytogenetics in Astyanax (Characiformes: Characidae) with focus on the cytotaxonomy of the group Neotropical Ichthyology
Tenório,Renata Cristina Claudino de Oliveira; Vitorino,Carla de Andrade; Souza,Issakar Lima; Oliveira,Claudio; Venere,Paulo Cesar.
Astyanax is a diverse group of Neotropical fishes, whose different forms occupy different environments. This great diversity is also reflected on cytogenetic aspects and molecular markers, which have repeatedly been demonstrated by cytogenetic studies. In order to characterize the karyotype of species of this genus, six species were studied: Astyanax altiparanae, A.argyrimarginatus, A. elachylepis, A. xavante, and two new species provisionally called Astyanax sp. and A. aff. bimaculatus. A detailed cytogenetic study based on conventional staining with Giemsa, AgNORs, C-banding, base-specific fluorochromes, and FISH using ribosomal genes 18S and 5S was conducted, aiming to understand some of the chromosomal mechanisms associated with the high...
Tipo: Info:eu-repo/semantics/article Palavras-chave: C-banding; CMA3 and DAPI fluorochromes; Fish chromosome; RDNA genes; NORs.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252013000300553
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Interindividual size heteromorphism of NOR and chromosomal location of 5S rRNA genes in Iheringichthys labrosus BABT
Carvalho,Rafael Augusto de; Dias,Ana Lúcia.
Twenty-five specimens of Iheringichthys labrosus from the Capivara Reservoir were analysed cytogenetically. AgNORs were detected in a pair of ST chromosomes, in the telomeric region of the long arm. Some individuals showed size heteromorphism of this region between homologous chromosomes. Treatment with CMA3 displayed GC-rich regions corresponding to the AgNOR pair, plus other fluorescent staining. In situ hybridization by fluorescence (FISH) with the 18S rDNA probe showed only one pair of stained chromosomes, confirming the heteromorphism observed with AgNO3 and CMA3 in some individuals. The 5S rDNA probe revealed telomeric staining on the long arm of a pair of chromosomes of the ST-A group, probably different from the NOR pair.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fish chromosome; AgNOR; In situ hibridization; Chromomycin A3; Heteromorphism.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000100017
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